INDOT workers are replacing signs along I-164 in Vanderburgh and Warrick counties with I-69 signs.
Officials say if the weather cooperates, all 29 miles should be renamed I-69 by the end of the year.
Businesses and other organizations are encouraged update their signage, maps and directions.
Bus routes have changed in Owensboro.
All routes leave and arrive at the main bus terminal at 430 Allen Street.
The Red, Blue, and White Routes come and go every 30 minutes, while the Brown, Orange, Yellow, Green, and Purple Routes operate every 45 minutes.
Officials say transfer points have been eliminated but will remain bus stops.